Creation and Genomic Analysis of Irradiation Hybrids in Populus.
Identifieur interne : 001937 ( Main/Exploration ); précédent : 001936; suivant : 001938Creation and Genomic Analysis of Irradiation Hybrids in Populus.
Auteurs : Matthew Zinkgraf [États-Unis] ; Kathy Haiby [États-Unis] ; Meric C. Lieberman [États-Unis] ; Luca Comai [États-Unis] ; Isabelle M. Henry [États-Unis] ; Andrew Groover [États-Unis]Source :
- Current protocols in plant biology [ 2379-8068 ] ; 2016.
Abstract
Establishing efficient functional genomic systems for creating and characterizing genetic variation in forest trees is challenging. Here we describe protocols for creating novel gene-dosage variation in Populus through gamma-irradiation of pollen, followed by genomic analysis to identify chromosomal regions that have been deleted or inserted in each F1 individual. Irradiated pollen is used in a controlled, interspecific cross to create F1 progeny that carry deletions and insertions of chromosomal regions. These insertions and deletions result in novel changes in gene dosage that in turn affect both qualitative and quantitative phenotypic traits. The protocols described here outline the processes involved in optimizing irradiation levels and performing controlled crosses, sowing seed and propagating seedlings, and genome-wide sequence-based analysis of deletions and insertions in the F1 progeny. The same approach could be applied to other vegetatively propagated species. © 2016 by John Wiley & Sons, Inc.
DOI: 10.1002/cppb.20025
PubMed: 30775870
Affiliations:
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Lieberman</name><affiliation wicri:level="2"><nlm:affiliation>Genome Center, University of California Davis, California.</nlm:affiliation><country>États-Unis</country><placeName><region type="state">Californie</region></placeName><wicri:cityArea>Genome Center, University of California Davis</wicri:cityArea></affiliation></author><author><name sortKey="Comai, Luca" sort="Comai, Luca" uniqKey="Comai L" first="Luca" last="Comai">Luca Comai</name><affiliation wicri:level="2"><nlm:affiliation>Department of Plant Biology, University of California, Davis, California.</nlm:affiliation><country>États-Unis</country><placeName><region type="state">Californie</region></placeName><wicri:cityArea>Department of Plant Biology, University of California, Davis</wicri:cityArea></affiliation><affiliation wicri:level="2"><nlm:affiliation>Genome Center, University of California Davis, California.</nlm:affiliation><country>États-Unis</country><placeName><region type="state">Californie</region></placeName><wicri:cityArea>Genome Center, University of California Davis</wicri:cityArea></affiliation></author><author><name sortKey="Henry, Isabelle M" sort="Henry, Isabelle M" uniqKey="Henry I" first="Isabelle M" last="Henry">Isabelle M. Henry</name><affiliation wicri:level="2"><nlm:affiliation>Department of Plant Biology, University of California, Davis, California.</nlm:affiliation><country>États-Unis</country><placeName><region type="state">Californie</region></placeName><wicri:cityArea>Department of Plant Biology, University of California, Davis</wicri:cityArea></affiliation><affiliation wicri:level="2"><nlm:affiliation>Genome Center, University of California Davis, California.</nlm:affiliation><country>États-Unis</country><placeName><region type="state">Californie</region></placeName><wicri:cityArea>Genome Center, University of California Davis</wicri:cityArea></affiliation></author><author><name sortKey="Groover, Andrew" sort="Groover, Andrew" uniqKey="Groover A" first="Andrew" last="Groover">Andrew Groover</name><affiliation wicri:level="2"><nlm:affiliation>U.S. Forest Service, Pacific Southwest Research Station, Davis, California.</nlm:affiliation><country>États-Unis</country><placeName><region type="state">Californie</region></placeName><wicri:cityArea>U.S. Forest Service, Pacific Southwest Research Station, Davis</wicri:cityArea></affiliation><affiliation wicri:level="2"><nlm:affiliation>Department of Plant Biology, University of California, Davis, California.</nlm:affiliation><country>États-Unis</country><placeName><region type="state">Californie</region></placeName><wicri:cityArea>Department of Plant Biology, University of California, Davis</wicri:cityArea></affiliation></author></analytic><series><title level="j">Current protocols in plant biology</title><idno type="eISSN">2379-8068</idno><imprint><date when="2016" type="published">2016</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Establishing efficient functional genomic systems for creating and characterizing genetic variation in forest trees is challenging. Here we describe protocols for creating novel gene-dosage variation in Populus through gamma-irradiation of pollen, followed by genomic analysis to identify chromosomal regions that have been deleted or inserted in each F1 individual. Irradiated pollen is used in a controlled, interspecific cross to create F1 progeny that carry deletions and insertions of chromosomal regions. These insertions and deletions result in novel changes in gene dosage that in turn affect both qualitative and quantitative phenotypic traits. The protocols described here outline the processes involved in optimizing irradiation levels and performing controlled crosses, sowing seed and propagating seedlings, and genome-wide sequence-based analysis of deletions and insertions in the F1 progeny. The same approach could be applied to other vegetatively propagated species. © 2016 by John Wiley & Sons, Inc.</div></front></TEI><pubmed><MedlineCitation Status="PubMed-not-MEDLINE" Owner="NLM"><PMID Version="1">30775870</PMID><DateRevised><Year>2019</Year><Month>11</Month><Day>20</Day></DateRevised><Article PubModel="Print"><Journal><ISSN IssnType="Electronic">2379-8068</ISSN><JournalIssue CitedMedium="Internet"><Volume>1</Volume><Issue>2</Issue><PubDate><Year>2016</Year><Month>Mar</Month></PubDate></JournalIssue><Title>Current protocols in plant biology</Title><ISOAbbreviation>Curr Protoc Plant Biol</ISOAbbreviation></Journal><ArticleTitle>Creation and Genomic Analysis of Irradiation Hybrids in Populus.</ArticleTitle><Pagination><MedlinePgn>431-450</MedlinePgn></Pagination><ELocationID EIdType="doi" ValidYN="Y">10.1002/cppb.20025</ELocationID><Abstract><AbstractText>Establishing efficient functional genomic systems for creating and characterizing genetic variation in forest trees is challenging. Here we describe protocols for creating novel gene-dosage variation in Populus through gamma-irradiation of pollen, followed by genomic analysis to identify chromosomal regions that have been deleted or inserted in each F1 individual. Irradiated pollen is used in a controlled, interspecific cross to create F1 progeny that carry deletions and insertions of chromosomal regions. These insertions and deletions result in novel changes in gene dosage that in turn affect both qualitative and quantitative phenotypic traits. The protocols described here outline the processes involved in optimizing irradiation levels and performing controlled crosses, sowing seed and propagating seedlings, and genome-wide sequence-based analysis of deletions and insertions in the F1 progeny. The same approach could be applied to other vegetatively propagated species. © 2016 by John Wiley & Sons, Inc.</AbstractText><CopyrightInformation>Copyright © 2016 John Wiley & Sons, Inc.</CopyrightInformation></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Zinkgraf</LastName><ForeName>Matthew</ForeName><Initials>M</Initials><AffiliationInfo><Affiliation>U.S. Forest Service, Pacific Southwest Research Station, Davis, California.</Affiliation></AffiliationInfo><AffiliationInfo><Affiliation>Department of Computer Science, University of California, Davis, California.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Haiby</LastName><ForeName>Kathy</ForeName><Initials>K</Initials><AffiliationInfo><Affiliation>Greenwood Resources, Portland, Oregon.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Lieberman</LastName><ForeName>Meric C</ForeName><Initials>MC</Initials><AffiliationInfo><Affiliation>Genome Center, University of California Davis, California.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Comai</LastName><ForeName>Luca</ForeName><Initials>L</Initials><AffiliationInfo><Affiliation>Department of Plant Biology, University of California, Davis, California.</Affiliation></AffiliationInfo><AffiliationInfo><Affiliation>Genome Center, University of California Davis, California.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Henry</LastName><ForeName>Isabelle M</ForeName><Initials>IM</Initials><AffiliationInfo><Affiliation>Department of Plant Biology, University of California, Davis, California.</Affiliation></AffiliationInfo><AffiliationInfo><Affiliation>Genome Center, University of California Davis, California.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Groover</LastName><ForeName>Andrew</ForeName><Initials>A</Initials><AffiliationInfo><Affiliation>U.S. Forest Service, Pacific Southwest Research Station, Davis, California.</Affiliation></AffiliationInfo><AffiliationInfo><Affiliation>Department of Plant Biology, University of California, Davis, California.</Affiliation></AffiliationInfo></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType></PublicationTypeList></Article><MedlineJournalInfo><Country>United States</Country><MedlineTA>Curr Protoc Plant Biol</MedlineTA><NlmUniqueID>101685882</NlmUniqueID></MedlineJournalInfo><KeywordList Owner="NOTNLM"><Keyword MajorTopicYN="N">Populus
</Keyword><Keyword MajorTopicYN="N">forest trees</Keyword><Keyword MajorTopicYN="N">gene dosage</Keyword><Keyword MajorTopicYN="N">genomics</Keyword><Keyword MajorTopicYN="N">mutagenesis</Keyword></KeywordList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="entrez"><Year>2019</Year><Month>2</Month><Day>19</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="pubmed"><Year>2016</Year><Month>3</Month><Day>1</Day><Hour>0</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2016</Year><Month>3</Month><Day>1</Day><Hour>0</Hour><Minute>1</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">30775870</ArticleId><ArticleId IdType="doi">10.1002/cppb.20025</ArticleId></ArticleIdList></PubmedData></pubmed><affiliations><list><country><li>États-Unis</li></country><region><li>Californie</li><li>Oregon</li></region></list><tree><country name="États-Unis"><region name="Californie"><name sortKey="Zinkgraf, Matthew" sort="Zinkgraf, Matthew" uniqKey="Zinkgraf M" first="Matthew" last="Zinkgraf">Matthew Zinkgraf</name></region><name sortKey="Comai, Luca" sort="Comai, Luca" uniqKey="Comai L" first="Luca" last="Comai">Luca Comai</name><name sortKey="Comai, Luca" sort="Comai, Luca" uniqKey="Comai L" first="Luca" last="Comai">Luca Comai</name><name sortKey="Groover, Andrew" sort="Groover, Andrew" uniqKey="Groover A" first="Andrew" last="Groover">Andrew Groover</name><name sortKey="Groover, Andrew" sort="Groover, Andrew" uniqKey="Groover A" first="Andrew" last="Groover">Andrew Groover</name><name sortKey="Haiby, Kathy" sort="Haiby, Kathy" uniqKey="Haiby K" first="Kathy" last="Haiby">Kathy Haiby</name><name sortKey="Henry, Isabelle M" sort="Henry, Isabelle M" uniqKey="Henry I" first="Isabelle M" last="Henry">Isabelle M. Henry</name><name sortKey="Henry, Isabelle M" sort="Henry, Isabelle M" uniqKey="Henry I" first="Isabelle M" last="Henry">Isabelle M. Henry</name><name sortKey="Lieberman, Meric C" sort="Lieberman, Meric C" uniqKey="Lieberman M" first="Meric C" last="Lieberman">Meric C. Lieberman</name><name sortKey="Zinkgraf, Matthew" sort="Zinkgraf, Matthew" uniqKey="Zinkgraf M" first="Matthew" last="Zinkgraf">Matthew Zinkgraf</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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